Reporter

Part:BBa_K945005:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-27)


Pichia pastoris optimized His-GFP-PTS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1144
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1144
    Illegal NgoMIV site found at 21
    Illegal NgoMIV site found at 1118
    Illegal AgeI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 1095


Design Notes

During codon optimization, restriction sites inside the coding sequence had to be avoided for it to be compatible with assembly standards.


Source

This sequence is a modification and optimization of the original wild-type GFP found in Aequorea victoria.

References